An overview of adding ROCK and KSR with trehalose to a low glycerol Tris-based semen extender

It is known that livestock animal semen very sensitive to cold shock during freezing processes, and this sensitivity directly affects post-thaw progressive motility, mitochondrial membrane potential, sperm nuclear DNA integrity in vitro spermatological parameters such as plasma acrosome integrity, fertility. In addition, with the sudden decrease total antioxidant level of after thawing, cells are insufficient tolerate their damage. As a result, significant losses occur For reason, researches on farm animals include processing; (cryopreservation/cryogenic damage) - thawing methods extenders, added antioxidants, mechanisms action metabolic pathways these antioxidants physiological has been explained low dose glycerol (trehalose increase cryoprotectant effect) extender semen, Knockout Serum Replacement (KSR) Rho-associated coiled-coil kinase (ROCK), which additives, can quality frozen thawed semen.